Special Lab Electron- and Laserscanning Microscopy

In modern neuroscience, imaging techniques at different scales play a central role in the elucidation of structure-function relationships. At the same time, high-resolution imaging is a rapidly evolving research field for the development of new technologies.

Therefore, the delivery of high-resolution microscopy infrastructure and imaging software to LIN's scientific departments and research groups, including consulting, training and service, is one of the key tasks of the Special Lab ELMI. In 2016/17 our infrastructure comprised among others: Confocal Laser Scanning Microscopes, Lightsheet Microscopes, STED, FLIM and a Transmission Electron Microscope.

In addition, the Special Lab ELMI carries out its own research projects on functional neuromorphology like the analysis of dynamic processes in pre- and postsynaptic structures and conducts methodological developments of high-resolution imaging procedures.

Based on the idea that imaging on different scales may bridge research between groups of LIN and, thus, plays a pivotal role for the implementation of the institute concept, the establishment of an overall CNI core facility was actively supported by the Special Labs NIB and ELMI.

  • Head

    Head

    Werner Zuschratter has been head of the special lab since 1992 and is also one of the coordinators of CoreFacility NeuroImaging (CNI).

    Our research interests focus on the analysis of structure-function relationships and the dynamics of biochemical, molecular biological and cell biological processes, especially in neurons and their synapses. In addition, we are researching the development of new technologies for functional imaging.

  • Members

    Members

    Head  
    Dr. Werner Zuschratter+49-391-6263-92441werner.zuschratter@lin-magdeburg.de
    Scientific staff members  
    Dr. Heide Faber-Zuschratter+49-391-6263-92391heidi.faber-zuschratter@lin-magdeburg.de
    Oliver Kobler+49-391-6263-93221oliver.kobler@lin-magdeburg.de
    Torsten Stöter (CNI)+49-391-6263-92171torsten.stoeter@lin-magdeburg.de
    André Weber+49-391-6263-92391andre.weber@lin-magdeburg.de
    Technical staff member  
    Sybille Tschorn+49-391-6263-93141sybille.tschorn@lin-magdeburg.de
    Guests  
    Dr. Yuri Prokazov  
    Evgeny Turbin  

     

  • Projects

    Projects

    Our mission is to offer its users a collection of state-of-the-art light and electron microscopy equipment and accessory services. In general, the activities of SL ELMI can be divided into three main parts:

    • advanced fluorescence microscopy/spectroscopy,
    • correlated light and transmission electron microscopy
    • 3D image processing and analysis.

     

    The light microscopy equipment includes Wide-field Fluorescence (WF) and Confocal Laserscanning Microscopes (CLSM) to image both living and fixed samples. In addition, the facility provides Total Internal Reflection Fluorescence Microscopy (TIRF), Lightsheet Microscopy (LS) and instrumentation for Fluorescence Lifetime Imaging (FLIM) based on Time-Correlated-Single-Photon-Counting (TCSPC), which directly examines protein complexes and molecular interactions via Fluorescence Resonance Energy Transfer (FRET). For high-resolution fluorescence imaging below the diffraction limit of visible light SL ELMI runs two STED-microscopes, which allow to explore colocalisations of macromolecular complexes in neurons and synapses.

    As a unique technology, we have ultra-sensitive time-resolved wide field detectors for FLIM and FRET measurements developed within the framework of the BMBF-funded collaborative project TCam4Life. These camera systems have been marketed since 2018 by the BMWI-funded start-up Photonscore GmbH under the name "LINCam".

  • Methods

    Methods

    • Confocal laserscanning microscopy
    • Electronmicroscopy
    • Fluorescence lifetime imaging microscopy & spectroscopy (FLIM)
    • Förster Resonance Energy Transfer (FRET)
    • Histochemistry & Immunocytochemistry
    • Lightsheet Microscopy
    • Live Cell Imaging
    • Metabolic Imaging (NADH)
    • Metal Induced Energy Transfer (MIET)
    • Time correlated single Photon counting (TCSPC)
    • Total Internal Reflection Fluorescence Microscopy (TIRF)

     

  • Third Party Funds

    Third Party Funds

    2010-2021
    CRC 854: Molecular organisation of cellular communication within the immune system
    TP Z01
    http://www.sfb854.de/


    2012-2019
    DFG Core Facility
    https://cni.lin-magdeburg.de

     

     

  • Publications

    Publications

    2019

    Leschik J, Eckenstaler R, Endres T, Munsch T, Edelmann E, Richter K, Kobler O, Fischer K-D, Zuschratter W, Brigadski T, Lutz B, Lessmann V. 2019. Prominent Postsynaptic and Dendritic Exocytosis of Endogenous BDNF Vesicles in BDNF-GFP Knock-in Mice. Molecular Neurobiology. https://doi.org/10.1007/s12035-019-1551-0

    Döring M, Blees H, Koller N, Tischer-Zimmermann S, Müsken M, Henrich F, Becker J, Grabski E, Wang J, Janssen H, Zuschratter W, Neefjes J, Klawonn F, Eiz-Vesper B, Tampé R, Kalinke U. 2019. Modulation of TAP-dependent antigen compartmentalization during human monocyte-to-DC differentiation. Blood advances. 3(6):839-850. https://doi.org/10.1182/bloodadvances.2018027268

     

    2018

    Bulitta B, Zuschratter W, Bernal I, Bruder D, Klawonn F, von Bergen M, Garritsen HSP, Jänsch L. 2018. Proteomic definition of human mucosal-associated invariant T cells determines their unique molecular effector phenotype. European Journal of Immunology. 48(8):1336-1349. https://doi.org/10.1002/eji.201747398

    Konugolu Venkata Sekar S, Mosca S, Tannert S, Valentini G, Martelli F, Binzoni T, Prokazov Y, Turbin E, Zuschratter W, Erdmann R, Pifferi A. 2018. Time domain diffuse Raman spectrometer based on a TCSPC camera for the depth analysis of diffusive media. Optics Letters. 43(9):2134-2137. https://doi.org/10.1364/OL.43.002134

    Saumweber T, Rohwedder A, Schleyer M, Eichler K, Chen Y-C, Aso Y, Cardona A, Eschbach C, Kobler O, Voigt A, Durairaja A, Mancini N, Zlatic M, Truman JW, Thum A, Gerber B. 2018. Functional architecture of reward learning in mushroom body extrinsic neurons of larval Drosophila. Nature Communications. 9(1). https://doi.org/10.1038/s41467-018-03130-1

    Mikhaylova M, Bär J, van Bommel B, Schätzle P, YuanXiang PA, Raman R, Hradsky J, Konietzny A, Loktionov EY, Reddy PP, Lopez-Rojas J, Spilker C, Kobler O, Raza SA, Stork O, Hoogenraad CC, Kreutz MR. 2018. Caldendrin Directly Couples Postsynaptic Calcium Signals to Actin Remodeling in Dendritic Spines. Neuron. 97(5):1110-1125.e14. https://doi.org/10.1016/j.neuron.2018.01.046

    Sekar SKV, Mosca S, Valentini G, Zuschratter W, Erdmann R, Pifferi A. 2018. Compact time domain diffuse raman instrumentation based on a TCSPC camera for depth probing of diffusive media. In Optical Tomography and Spectroscopy, OTS 2018. OSA - The Optical Society. https://doi.org/10.1364/OTS.2018.OTu4D.2

     

    2017

    Zhao B, Meka DP, Scharrenberg R, König T, Schwanke B, Kobler O, Windhorst S, Kreutz MR, Mikhaylova M, Calderon De Anda F. 2017. Microtubules Modulate F-actin Dynamics during Neuronal Polarization. Scientific Reports. 7(1). Available from: 10.1038/s41598-017-09832-8

    Wagner M, Baer C, Zuschratter W, Riek-Burchardt M, Deffge C, Weinert S, Lee JC, Braun-Dullaeus RC, Herold J. 2017. Intravital Microscopy of Monocyte Homing and Tumor-Related Angiogenesis in a Murine Model of Peripheral Arterial Disease. Journal of Visualized Experiments. (126). Available from: 10.3791/56290

    Korthals M, Langnaese K, Smalla KH, Kähne T, Herrera-Molina R, Handschuh J, Lehmann AC, Mamula D, Naumann M, Seidenbecher C, Zuschratter W, Tedford K, Gundelfinger ED, Montag D, Fischer KD, Thomas U. 2017. A complex of Neuroplastin and Plasma Membrane Ca2+ ATPase controls T cell activation. Scientific Reports. 7(1). Available from: 10.1038/s41598-017-08519-4

    Herrera-Molina R, Mlinac-Jerkovic K, Ilic K, Stöber F, Vemula SK, Sandoval M, Milosevic NJ, Simic G, Smalla KH, Goldschmidt J, Bognar SK, Montag D. 2017. Neuroplastin deletion in glutamatergic neurons impairs selective brain functions and calcium regulation: Implication for cognitive deterioration. Scientific Reports. 7(1). Available from: 10.1038/s41598-017-07839-9

    Maldonado H, Calderon C, Burgos-Bravo F, Kobler O, Zuschratter W, Ramirez O, Härtel S, Schneider P, Quest AFG, Herrera-Molina R, Leyton L. 2017. Astrocyte-to-neuron communication through integrin-engaged Thy-1/CBP/Csk/Src complex triggers neurite retraction via the RhoA/ROCK pathway. Biochimica et Biophysica Acta - Molecular Cell Research. 1864(2):243-254. Available from: 10.1016/j.bbamcr.2016.11.006

    Philipsen L, Reddycherla AV, Hartig R, Gumz J, Kästle M, Kritikos A, Poltorak MP, Prokazov Y, Turbin E, Weber A, Zuschratter W, Schraven B, Simeoni L, Müller AJ. 2017. De novo phosphorylation and conformational opening of the tyrosine kinase Lck act in concert to initiate T cell receptor signaling. Science Signaling. 10(462). Available from: 10.1126/scisignal.aaf4736

    Bhattacharya S, Herrera-Molina R, Sabanov V, Ahmed T, Iscru E, Stöber F, Richter K, Fischer KD, Angenstein F, Goldschmidt J, Beesley PW, Balschun D, Smalla KH, Gundelfinger ED, Montag D. 2017. Genetically induced retrograde amnesia of associative memories after neuroplastin ablation. Biological Psychiatry. 81(2):124-135. Available from: 10.1016/j.biopsych.2016.03.2107

    Weber A, Prokazov Y, Zuschratter W, Hauser MJB. 2017. From synchronised to desynchronised glycolytic oscillations in individual yeast cells. In Complexity and Synergetics. Springer International Publishing. pp. 239-254. Available from: 10.1007/978-3-319-64334-2_19

    Klasvogt S, Zuschratter W, Schmidt A, Kröber A, Vorwerk S, Wolter R, Isermann B, Wimmers K, Rothkötter HJ, Nossol C. 2017. Air-liquid interface enhances oxidative phosphorylation in intestinal epithelial cell line IPEC-J2. Cell death discovery. 3:17001. Available from: 10.1038/cddiscovery.2017.1

    Junghans C, Vukojević V, Tavraz NN, Maksimov EG, Zuschratter W, Schmitt FJ, Friedrich T. 2017. Disruption of Ankyrin B and Caveolin-1 Interaction Sites Alters Na+,K+-ATPase Membrane Diffusion. Biophysical Journal. 113(10):2249-2260. Available from: 10.1016/j.bpj.2017.08.053

     

    2016

    Medunjanin S, Schleithoff L, Fiegehenn C, Weinert S, Zuschratter W, Braun-Dullaeus RC. 2016. GSK-3β controls NF-kappaB activity via IKKγ/NEMO. Scientific Reports. 6. Available from: 10.1038/srep38553

    Bär J, Kobler O, Van Bommel B, Mikhaylova M. 2016. Periodic F-actin structures shape the neck of dendritic spines. Scientific Reports. 6. Available from: 10.1038/srep37136

    Ferrando-May E, Hartmann H, Reymann J, Ansari N, Utz N, Fried HU, Kukat C, Peychl J, Liebig C, Terjung S, Laketa V, Sporbert A, Weidtkamp-Peters S, Schauss A, Zuschratter W, Avilov S. 2016. Advanced light microscopy core facilities: Balancing service, science and career. Microscopy Research and Technique. 79(6):463-479. Available from: 10.1002/jemt.22648

    Mikhaylova M, Bera S, Kobler O, Frischknecht R, Kreutz MR. 2016. A Dendritic Golgi Satellite between ERGIC and Retromer. Cell Reports. 14(2):189-199. Available from: 10.1016/j.celrep.2015.12.024

    Tran VL, Génot V, Audibert J-F, Prokazov Y, Turbin E, Zuschratter W, Kim H-J, Jung C, Park SY, Pansu RB. 2016. Nucleation and growth during a fluorogenic precipitation in a micro-flow mapped by fluorescence lifetime microscopy. New Journal of Chemistry. 40(5):4601-4605.

     

    2015

    Sokoll S, Prokazov Y, Hanses M, Biermann B, Tönnies K, Heine M. 2015. Fast three-dimensional single-particle tracking in natural brain tissue. Biophysical Journal. 109(7):1463-1471. Available from: 10.1016/j.bpj.2015.07.047

    Nossol C, Barta-Böszörményi A, Kahlert S, Zuschratter W, Faber-Zuschratter H, Reinhardt N, Ponsuksili S, Wimmers K, Diesing A, Rothkötter HJ. 2015. Comparing two intestinal porcine epithelial cell lines (IPECs): Morphological differentiation, function and metabolism. PLoS ONE. 10(7). Available from: 10.1371/journal.pone.0132323

    Erdmann I, Marter K, Kobler O, Niehues S, Abele J, Müller A, Bussmann J, Storkebaum E, Ziv T, Thomas U, Dieterich DC. 2015. Cell-selective labelling of proteomes in Drosophila melanogaster. Nature Communications. 6. Available from: 10.1038/ncomms8521

    Medunjanin S, Daniel JM, Weinert S, Dutzmann J, Burgbacher F, Brecht S, Bruemmer D, Kähne T, Naumann M, Sedding DG, Zuschratter W, Braun-Dullaeus RC. 2015. DNA-dependent protein kinase (DNA-PK) permits vascular smooth muscle cell proliferation through phosphorylation of the orphan nuclear receptor NOR1. Cardiovascular Research. 106(3):488-497. Available from: 10.1093/cvr/cvv126

    Fidzinski P, Korotkova T, Heidenreich M, Maier N, Schuetze S, Kobler O, Zuschratter W, Schmitz D, Ponomarenko A, Jentsch TJ. 2015. KCNQ5 K+ channels control hippocampal synaptic inhibition and fast network oscillations. Nature Communications. 6. Available from: 10.1038/ncomms7254

     

    2014

    Prokazov Y, Turbin E, Weber A, Hartig R, Zuschratter W. 2014. Position sensitive detector for fluorescence lifetime imaging. Journal of Instrumentation. 9(12). Available from: 10.1088/1748-0221/9/12/C12015

    Beesley PW, Herrera-Molina R, Smalla KH, Seidenbecher C. 2014. The Neuroplastin adhesion molecules: key regulators of neuronal plasticity and synaptic function. Journal of Neurochemistry. 131(3):268-283. Available from: 10.1111/jnc.12816

    Koch N, Kobler O, Thomas U, Qualmann B, Kessels MM. 2014. Terminal axonal arborization and synaptic bouton formation critically rely on Abp1 and the Arp2/3 complex. PLoS ONE. 9(5). Available from: 10.1371/journal.pone.0097692

    Herrera Molina R, Sarto-Jackson I, Montenegro-Venegas C, Heine M, Smalla KH, Seidenbecher CI, Beesley PW, Gundelfinger ED, Montag D. 2014. Structure of excitatory synapses and GABA<inf>a</inf> receptor localization at inhibitory synapses are regulated by neuroplastin-65. Journal of Biological Chemistry. 289(13):8973-8988. Available from: 10.1074/jbc.M113.514992

    Mikhaylova M, Karpova A, Bär J, Bethge P, YuanXiang P, Chen Y, Zuschratter W, Behnisch T, Kreutz MR. 2014. Cellular distribution of the NMDA-receptor activated synapto-nuclear messenger Jacob in the rat brain. Brain Structure and Function. 219(3):843-860. Available from: 10.1007/s00429-013-0539-1

    Hartig R, Prokazov Y, Turbin E, Zuschratter W. 2014. Wide-Field fluorescence lifetime imaging with multi-anode detectors. In Fluorescence Spectroscopy and Microscopy: Methods and Protocols. Humana Press Inc. pp. 457-480. (Methods in Molecular Biology). Available from: 10.1007/978-1-62703-649-8_20

     

    2013

    Diesing AK, Nossol C, Faber-Zuschratter H, Zuschratter W, Renner L, Sokolova O, Naumann M, Rothkötter HJ. 2013. Rapid interaction of helicobacter pylori with microvilli of the polar human gastric epithelial cell line NCI-N87. Anatomical Record. 296(12):1800-1805. Available from: 10.1002/ar.22818

    Sandoval M, Luarte A, Herrera-Molina R, Varas-Godoy M, Santibáñez M, Rubio FJ, Smit AB, Gundelfinger ED, Li KW, Smalla KH, Wyneken U. 2013. The glycolytic enzyme aldolase C is up-regulated in rat forebrain microsomes and in the cerebrospinal fluid after repetitive fluoxetine treatment. Brain Research. 1520:1-14. Available from: 10.1016/j.brainres.2013.04.049

    Sakaba T, Kononenko NL, Bacetic J, Pechstein A, Schmoranzer J, Yao L, Barth H, Shupliakov O, Kobler O, Aktories K, Haucke V. 2013. Fast neurotransmitter release regulated by the endocytic scaffold intersectin. Proceedings of the National Academy of Sciences of the United States of America. 110(20):8266-8271. Available from: 10.1073/pnas.1219234110

    Karpova A, Mikhaylova M, Bera S, Bär J, Reddy PP, Behnisch T, Rankovic V, Spilker C, Bethge P, Sahin J, Kaushik R, Zuschratter W, Kähne T, Naumann M, Gundelfinger ED, Kreutz MR. 2013. Encoding and transducing the synaptic or extrasynaptic origin of NMDA receptor signals to the nucleus. Cell. 152(5):1119-1133. Available from: 10.1016/j.cell.2013.02.002

    Stirnweiss A, Hartig R, Gieseler S, Lindquist JA, Reichardt P, Philipsen L, Simeoni L, Poltorak M, Merten C, Zuschratter W, Prokazov Y, Paster W, Stockinger H, Harder T, Gunzer M, Schraven B. 2013. T cell activation results in conformational changes in the Src family kinase Lck to induce its activation. Science Signaling. 6(263). Available from: 10.1126/scisignal.2003607

    Vielhaber S, Debska-Vielhaber G, Peeva V, Schoeler S, Kudin AP, Minin I, Schreiber S, Dengler R, Kollewe K, Zuschratter W, Kornblum C, Zsurka G, Kunz WS. 2013. Mitofusin 2 mutations affect mitochondrial function by mitochondrial DNA depletion. Acta Neuropathologica. 125(2):245-256. Available from: 10.1007/s00401-012-1036-y

    Schoonderwoert V, Dijkstra R, Luckinavicius G, Kobler O, van der Voort H. 2013. Huygens STED Deconvolution Increases Signal-to-Noise and Image Resolution towards 22 nm. Microscopy Today. 21(06):38 - 44. Available from: 10.1017/S1551929513001089

    Hradsky J, Mikhaylova M, Karpova A, Kreutz MR, Zuschratter W. 2013. Super-resolution microscopy of the neuronal calcium-binding proteins calneuron-1 and caldendrin. In Methods in Molecular Biology. pp. 147-169. (Methods in Molecular Biology). Available from: 10.1007/978-1-62703-230-8_10

     

    2012

    Weber A, Prokazov Y, Zuschratter W, Hauser MJB. 2012. Desynchronisation of Glycolytic Oscillations in Yeast Cell Populations. PLoS ONE. 7(9). Available from: 10.1371/journal.pone.0043276

    Lovell J, Brosch M, Budinger E, Goldschmidt J, Scheich H, Tschorn S, Wendt B, Zuschratter W. 2012. Scanning and Transmission Electron Microscope Examination of Cochlea Hair and Pillar Cells from the Ear of the Mongolian Gerbil (Meriones unguiculatus). Anatomy and Physiology. 2(3).

    Herrera-Molina R, Frischknecht R, Maldonado H, Seidenbecher CI, Gundelfinger ED, Hetz C, de la Luz Aylwin M, Schneider P, Quest AFG, Leyton L. 2012. Astrocytic α vβ 3 integrin inhibits neurite outgrowth and promotes retraction of neuronal processes by clustering thy-1. PLoS ONE. 7(3). Available from: 10.1371/journal.pone.0034295

     

    2011

    Michaluk P, Wawrzyniak M, Alot P, Szczot M, Wyrembek P, Mercik K, Medvedev N, Wilczek E, De Roo M, Zuschratter W, Muller D, Wilczynski GM, Mozrzymas JW, Stewart MG, Kaczmarek L, Wlodarczyk J. 2011. Influence of matrix metalloproteinase MMP-9 on dendritic spine morphology. Journal of Cell Science. 124(19):3369-3380. Available from: 10.1242/jcs.090852

    Vitali M, Picazo F, Prokazov Y, Duci A, Turbin E, Götze C, Llopis J, Hartig R, Visser AJWG, Zuschratter W. 2011. Wide-Field multi-parameter FLIM: Long-term minimal invasive observation of proteins in living cells. PLoS ONE. 6(2). Available from: 10.1371/journal.pone.0015820

     

    2010

    Wittenbrink N, Weber TS, Klein A, Weiser AA, Zuschratter W, Sibila M, Schuchhardt J, Or-Guil M. 2010. Broad volume distributions indicate nonsynchronized growth and suggest sudden collapses of germinal center B cell populations. Journal of Immunology. 184(3):1339-1347. Available from: 10.4049/jimmunol.0901040

  • Teaching

    Teaching

    We are involved in the education of students of the master's program "Integrative Neuroscience".


    Members of our lab teach MicroImaging and offer the following internship opportunities:

    • MicroImaging (Lecture)
    • MicroImaging (Practical Course)


    In addition, we conduct workshops on image analysis and, in cooperation with the Neuroscientific Society (NWG), hold annual courses on the functional organization of synapses within the framework of the CNI CoreFacility.

  • Awards

    Awards

    • 2013: 1st place at the Hugo-Junkers-Award for Research and Innovation, Category: Basic Research (Saxony-Anhalt)
       
    • 2014: Cluster Award Life Sciences IQ Mitteldeutschland
       
    • 2017: 1st place at the Hugo-Junkers-Award for Research and Innovation, Category: Applied Research (Saxony-Anhalt)
       
    • 2019: Nomination for the German Innovation Award (Deutscher Innovationspreis)

     

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